For example, the intensity of Rayleigh scattering at 400 nm in the blue part of the visible spectrum is higher by a factor of 9. Depending on the exact sample characteristics and the required measurement the sample holder is selected from disposable size/zeta cell, flow cell, high concentration cell, flat surface cell and low conductivity cell. Dynamic Light Scattering (DLS), sometimes referred to as Photon Correlation Spectroscopy or Quasi-Elastic Light Scattering, is a technique classically used for measuring the size of particles typically in the sub-micron region, dispersed in a liquid. Equation 4 indicates that the Rayleigh scattering intensity varies as the fourth power of the inverse wavelength of the incident light. These measurements are obtained directly by the Beckman Coulter Delsa Nano Submicron Particle analyzer. ![]() By calculating the auto correlation functions of the time dependent intensity fluctuations hydrodynamic diameter is obtained by particle light scattering while zeta potential is obtained using the electrophoretic light scattering and laser doppler method. In this technique, the fluctuations in time of scattered light from the particles in Brownian motion are measured. According to the Stokes-Einstein equation, the diffusion coefficient (D) is inversely proportional to the particle size (d). ![]() This motion causes particle diffusion through the medium. Particles suspended in liquids are in Brownian motion as a result of random collisions with solvent molecules. ![]() Light Scattering Measurements of Particles in SolutionÄynamic light scattering measurements are used to obtain the particle diameters and the surface charge of the nanoparticles in solution phase.
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